WITHDRAWN: Gene silencing by double-stranded RNA

Gene silencing by double-stranded RNA (Nobel Lecture).

Double-Stranded RNA Minireview as a Template for Gene Silencing

relieve the inhibition, suggesting micrococcal nuclease When double-stranded RNA (dsRNA) corresponding to was not merely competing for nucleic acid binding.) a sense and antisense sequence of an endogenous Further pieces of the puzzle are provided by beautiful mRNA is introduced into a cell, in organisms ranging work from a collaborative effort of Phil Zamore, Tom from trypanosomes to mice, the...

Double-Stranded RNA as a Template for Gene Silencing

relieve the inhibition, suggesting micrococcal nuclease When double-stranded RNA (dsRNA) corresponding to was not merely competing for nucleic acid binding.) a sense and antisense sequence of an endogenous Further pieces of the puzzle are provided by beautiful mRNA is introduced into a cell, in organisms ranging work from a collaborative effort of Phil Zamore, Tom from trypanosomes to mice, the...

Double-stranded RNA-mediated gene silencing in fission yeast.

Double-stranded RNA (dsRNA) can specifically inhibit gene expression in a variety of organisms by invoking post-transcriptional degradation of homologous mRNA. Here we show that dsRNA-mediated gene regulation also occurs in the fission yeast Schizosaccharomyces pombe. We present evidence that: (i) reporter gene silencing is significantly enhanced when additional non-coding sense RNA is co-expre...

Knockdown of LIM15/DMC1 in the mushroom Coprinus cinereus by double-stranded RNA-mediated gene silencing.

The basidiomycete Coprinus cinereus has many advantages as a model organism for studying sexual development and meiosis, but it has been difficult to investigate using reverse-genetics methods, such as gene disruption by homologous recombination. Here, gene repression by dsRNA-mediated gene silencing was tried as an alternative method for reverse-genetics studies. It was shown that transformati...